Incorporation of labelled precursors into the electrophoretic fractions of rat-liver ribonucleic acid
نویسندگان
چکیده
منابع مشابه
The effect of chick-liver ribonucleic acid on amino acid-incorporation systems from rat liver.
1. Rat-liver microsomes, ribonucleoprotein particles and a fraction mainly consisting of microsomal membranes were tested for their ability to incorporate amino acids into protein in the presence of ATP, GTP, phosphoenolpyruvate and pyruvate kinase. Addition of polyuridylic acid or of ribonucleic acid from rat-liver nuclei stimulated the incorporating activities. 2. These protein-synthesizing s...
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The role of nucleotides as precursors of ribonucleic acid in animal tissues has been well documented (l-4). ilside from the formation of polymers by the enzyme polynucleotide phosphorylase (5), only one preliminary report of net synthesis of polyribonucleotides in cell-free systems has been made (6). Hilmoe and Heppel (7) have demonstrated the phosphorolysis of added polyadenylic acid by a part...
متن کاملIncorporation of terminal ribonucleotides into soluble ribonucleic acid by a purified rat liver enzyme.
The incorporation of ribonucleotidee from ribonucleoside 5’-triphosphates into the terminal position of a soluble ribonucleic acid fraction has been reported by a number of laboratories (1, 2). Hecht, Zamecnic, Stephenson, and Scott (3) showed that s-RNA1 isolated from a “preincubated pH 5 fraction” of rat liver or ascites tumor cells could accept 2 cytidylate units per adenylate unit incorpora...
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Freshly explanted leukemic myeloblasts produce avian myeloblastosis virus (AMV) at a constant rate without any obvious cytopathic effect; therefore, subviral components are continually synthesized at a steady rate. The incorporation of various radioactive precursors into virions was monitored by determination of radioactivity in purified virus after density equilibrium sedimentation in preforme...
متن کاملThe incorporation of adenylic and cytidylic acids into ribonucleic acid.
Amino acid acceptor ribonucleic acid isolated from mammalian or bacterial sources is terminated by adenylic acid (l-4). A number of investigators have examined the enzymatic incorporation of adenosine phosphate and demonstrated that adenosine phosphate could be added to the end of ribonucleic acid chains (5-7). I f ATP containing Pa in the a-phosphate was used as the substrate and the product, ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1966
ISSN: 0006-2936
DOI: 10.1042/bj1000204